Memórias do Instituto Oswaldo Cruz (Vol.94(I): 229-238, 1999).

Mitochondrial DNA Variation of Triatoma infestans
Populations and its Implication on the Specific Status of T. melanosoma.

Fernando A Monteiro/*/ + , Ruben Pérez**, Francisco Panzera**, Jean-Pierre
Dujardin***, Cleber Galvão****, Dayse Rocha****, Francois Noireau****,
Chris Schofield*****, Charles B Beard

Centers for Disease Control and Prevention, 4770 Buford Hwy MS: F-22, 30341-3724 Atlanta, USA. *Depto de Genetica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil **Sección Genética Evolutiva, Facultad
de Ciencias, Montevideo, Uruguay ***Institut de Recherche pour le Development,La Paz, Bolivia. ****Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Departamento de Entomologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil *****London School of Hygiene and Tropical Medicine, London, UK

DNA sequence comparison of 412 base-pairs fragments of the mitochondrial cytochrome B gene was used to infer the genetic structure of nine geographical Triatoma infestans populations and their phylogenetic relationship with T. melanosoma and T. brasiliensis. T. infestans and T. melanosoma werecompared by morphometry, allozyme and cytogenetic analyses, as well as subjected to reciprocal crosses,in order to clarify the taxonomic status of the latter. No differences were found to distinguish the two species and the crosses between them yielded progeny. T. infestans populations presented four haplotypes that could be separated in two clusters: one formed by the samples from Bolivia (Andes and Chaco) and the other formed by samples from Argentina and Brazil. Silvatic and domestic T. infestans populations from Bolivia (Andes) were genetically identical.

Key words: Triatoma infestans - Triatoma melanosoma - mitochondrial DNA - cytogenetics - allozymes -phylogeny - molecular systematics - Chagas disease vectors